Journal: Journal of leukocyte biology

Article Title: Adipose tissue of human omentum is a major source of dendritic cells, which lose MHC Class II and stimulatory function in Crohn's disease.

doi: 10.1189/jlb.0905501

Figure Lengend Snippet: Fig. 5. Morphology of omental DC. (a) A typical DC from a control omentum is shown. (b) An example of immunogold staining of the veils of a DC. The original bars indicate 1 m. In controls, gold labeling was always 5 gold grains per cell; 10 grains was considered positive. (c) The types of myeloid cells identified in the omental cells from two control patients; the proportion positive for HLA-DR staining is shown in the solid portion of the histograms, and those negative are hatched. M , Macrophage. (d) The gold grains per cell for the surface DR-labeling in the two experiments performed are shown. No evidence of DR-labeling was seen in either of the two Crohn’s patients studied. (e) A light microscopy picture of a cluster of putative DC immunostained with CD11c on a frozen section of omentum from a Crohn’s patient showing the dark, positive-peroxidase staining, which was absent from control slides. Cell nuclei, which were stained with hematoxylin, were distinguishable, showing that the veils were associated with mononuclear cells in the section. (f) Control for e using serum instead of specific antibody and with only hematoxylin staining visible. (g) Frozen section of control omentum, showing two cells specifically labeled for CD83 (TRITC-labeled). (h) The same section as g with label specific for CD86 (FITC-labeled).

Article Snippet: To block nonspecific binding sites, sections were incubated with normal mouse serum for 1 h and then incubated with FITC-conjugated primary antibody to CD86, CD80, or CD40 (Serotec) for 4 h, washed in PBS, and mounted under cover-slips using 4 ,6-diamidino-2-phenylindole-conjugated mounting medium (Vector Laboratories).

Techniques: Control, Staining, Labeling, Light Microscopy

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Self-Healing Hydrogels and Cryogels from Biodegradable Polyurethane Nanoparticle Crosslinked Chitosan.

doi: 10.1002/advs.201901388

Figure Lengend Snippet: Figure 8. Foreign body reaction of CS-PU cryogels after rat subcutaneous implantation. A) Histology of H&E-stained sections after implantation for 14 d. The scale bar represents 500 µm. B) The extent of foreign body reaction could be revealed by the thickness of the fibrous capsule (white arrows) based on the histology. C) Immunofluorescent images (marker protein expression) of macrophages, stained by the mouse monoclonal anti-CD86 antibody for M1 macrophages (red), or mouse monoclonal anti-CD163 antibody for M2 macrophages (green). D) Quantification of M1 macrophage and M2 macrophage populations. Results are expressed as mean ± SD, N = 3. **p < 0.01, and **** p < 0.0001 among the indicated groups. PU (nonfunctionalized) films were used as the control.

Article Snippet: The tissue sections were treated with mouse anti-CD86 antibody (Bio-Rad, USA) for staining of M1 macrophages, or mouse anti-CD163 antibody (Bio-Rad, USA) for staining of M2 macrophages at 4 °C overnight.

Techniques: Staining, Marker, Expressing, Control

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Interaction of Coxiella burnetii Strains of Different Sources and Genotypes with Bovine and Human Monocyte-Derived Macrophages

doi: 10.3389/fcimb.2017.00543

Figure Lengend Snippet: Antibodies against MDM surface antigens used in this study.

Article Snippet: CD86 (FITC) , Human , BU63 (Biozol) , IgG1 , 1:222.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Interaction of Coxiella burnetii Strains of Different Sources and Genotypes with Bovine and Human Monocyte-Derived Macrophages

doi: 10.3389/fcimb.2017.00543

Figure Lengend Snippet: Effects of different C. burnetii strains on expression of different surface markers by bovine and human MDM. Cells were inoculated with C. burnetii strains (100 MOI for 1 h) or stimulated with E. coli LPS (6 μg/ml), harvested 24 h later and immunolabelled for surface markers CD40, CD80, CD86, MHCI, and MHCII. Results of 3–8 independent experiments per C. burnetii strain are shown as box and whisker plots ( * p ≤ 0.05; ** p ≤ 0.01). Strains are indicated by numbers as listed in Table .

Article Snippet: CD86 (FITC) , Human , BU63 (Biozol) , IgG1 , 1:222.

Techniques: Expressing, Whisker Assay